Magnetic Bead Method Soil DNA Extraction Kit
This product is suitable for DNA extraction from soil and other samples. The kit is based on superparamagnetic magnetic bead purification technology. During the extraction process, according to the ultra-high affinity of magnetic beads to nucleic acid under specific conditions and the characteristics of nucleic acid release when conditions are changed, the effect of rapid nucleic acid separation and purification is achieved. The rapid separation and purification process does not require the use of toxic phenol-chloroform extraction, nor does it require time-consuming alcohol precipitation. The entire extraction process takes only 90 to 120 minutes, especially for high-throughput workstations and other automated nucleic acid extraction equipment. The obtained DNA can be directly used in PCR, fluorescence quantification, microorganism detection and other experiments.
【 Product Components】
|
Product No. |
DC306-01（32T） |
DC306-02（64T） |
DC306-03（96T） |
|
Prepackaged deep well plate |
16T×2 plate |
16T×4 plate |
16T×6 plate |
|
Disposable magnetic sleeve |
2 packs×2 pcs/pack |
4 packs×2 pcs/pack |
6 packs×2 pcs/pack |
|
ALFA Lysis Buffer A |
30ml×1 bottle |
45 ml×1 bottle |
60 ml×1 bottle |
|
ALFA Lysis Buffer B |
4ml×1 bottle |
5ml×1 bottle |
6ml×1 bottle |
|
ALFA PI Buffer 1 |
10ml×1 bottle |
15ml×1 bottle |
20ml×1 bottle |
|
ALFA PI Buffer B |
8ml×1 bottle |
12ml×1 bottle |
15ml×1 bottle |
|
Glass Beads Tube I |
16T×2 packs |
16T×4 packs |
16T×6 packs |
【Storage conditions and expiration date】
This product is transported and stored at room temperature (15°C~25°C). The validity period is 12 months.
For long-term storage, it needs to be placed at 2~8℃. At low temperature, ALFA Lysis Buffer B may form the precipitate. A 37°C water bath is required to dissolve it completely.
【Preparations】
1. Automatic nucleic acid extraction instrument, constant temperature water bath
2. Before using the prepackaged deep-well plate nucleic acid extraction reagent, gently tap the deep-well plate vertically so that no reagents or magnetic beads remain on the parafilm.
【Sample Pre-treatment】
1. Add 0.25~0.50g of soil sample to the grinding tube (Glass Beads Tube I), then add 750μl ALFA Lysis Buffer A and 60μl ALFA Lysis Buffer B (900μl ALFA Lysis Buffer A and 70μl ALFA Lysis Buffer B are optional for dry soil), Vortex mixing at the highest speed for 10 minutes, or a grinder can be used for mixing (specific parameters are adjusted according to the model of the instrument).
2. Heat at 55°C for 15 minutes.
3. Centrifuge at 14,000×g for 5 minutes, transfer ~600μl of supernatant to a new centrifuge tube.
4. Add 250μl ALFA PI Buffer 1 to the supernatant, vortex to mix.
5. Place on ice or at 4ºC for 5 minutes and centrifuge at 14,000x g for 5 minutes.
6. Add 200μl Buffer PI B to the supernatant and mix well. Place on ice or at 4ºC for 5 minutes, and centrifuge at 14,000x g for 5 minutes. The supernatant is ready for use.
【Experimental steps】
- Deep-well plate reagent preparation
|
Hole position |
Add before use |
|
1/7 |
450μl soil supernatant |
|
2/8 |
450μl soil supernatant |
- Machine program parameters
- After the nucleic acid extractor runs for about 30 minutes, the nucleic acid extraction process is completed. Take out the matching deep-well plate, and transfer the liquid nucleic acid product extracted from the 6th/12th well for use. (See Note 1)
Note 1: The extracted liquid nucleic acid product should be used for qPCR assay as soon as possible. If not immediately available for testing, store at -20°C as soon as possible.
- Discard waste liquid and consumables according to standard procedures.
【 Manufacturer】
Company Name: Findrop Biosafety Technology (Guangzhou) Co., Ltd.
Address: Room 301, Building 2, No. 27 Yayingshi Road, Huangpu District, Guangzhou
Hotline: 400-9922230
【 Approval date of the manual】
Approval Date：May 12, 2022
V1.2 20220513
